Author Affiliations
Abstract
1 State Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), Shenzhen 518060, China
2 Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province & Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively. However, the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues. This paper introduces an iterative multi-photon adaptive compensation technique (IMPACT) into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues. It shows that 400 correction modes can be achieved within 5 min, which was mainly limited by the frame rate of a spatial light modulator. This system was used for high-resolution imaging of mice brain tissue and live zebrafish, further verifying its superior performance in imaging quality and photon accumulation speed.
adaptive optics iterative optimization two-photon fluorescence lifetime imaging microscopy wavefront correction Chinese Optics Letters
2024, 22(4): 041702
光通信研究
2024, 50(2): 22007301
1 中国科学院光电技术研究所自适应光学重点实验室,四川 成都 610209
2 中国科学院大学,北京 100049
针对较强天光背景下基于暗弱钠导星的大气波前畸变像差实时探测需求,本文提出了一种综合滤波的主动式波前探测技术,完成了对该技术的理论分析、参数设计及探测能力预估,并将该技术应用于传统哈特曼波前探测器,开展了基于钠导星的大气波前畸变像差探测实验。在约10 W/(m2·sr)的天光背景条件下,实现了基于钠导星同步采样大气波前畸变像差的实时探测。本工作对实际钠导星自适应光学系统应用的工作时段扩展进行了有益尝试。
自适应光学 钠导星 天光背景 波前探测
1 中国科学院自适应光学重点实验室,四川 成都 610209
2 中国科学院光电技术研究所,四川 成都 610209
3 中国科学院大学电子电气与通信工程学院,北京 100049
自适应光学(AO)技术是一种能够补偿大气湍流的有效手段,但由于存在系统固有时延,变形镜(DM)上的补偿波前滞后于实测畸变波前,导致AO技术对高时间频率大气湍流的校正效果明显下降。因此,开展大气湍流前向预测研究对于抵消AO系统固有时延、提升系统校正带宽具有重要的研究意义和应用价值。本文提出了一种基于注意力机制的AO波前时空预测网络,该网络同时考虑了大气湍流的时间与空间特征,可通过连续6帧先验波前斜率信息预测未来第2帧的波前斜率。在具有两帧延迟的AO系统仿真中,所提预测网络使得波前校正残差均方根(RMS)下降了约40%,并且在不同的大气湍流强度下均表现出稳定的预测精度,预测残差RMS仅为真实畸变波前RMS的5.00%。最后使用1 km激光大气传输系统采集的实验数据进行了测试,验证了开环斜率预测网络的有效性。
自适应光学 时延误差 注意力机制 波前预测
大连理工大学光电工程与仪器科学学院,辽宁 大连 116024
超分辨荧光显微镜突破了传统荧光显微镜的分辨率限制,使得人们能够在纳米量级分辨率下观察细胞和组织样品,极大地推动了生命科学的发展。在这一技术中,仪器和样品引入的像差均会导致空间分辨率降低,进而导致成像质量恶化。为此,人们引入了自适应光学技术,通过直接或间接的手段探测像差,再通过波前校正元件来校正像差,从而获得高质量的超分辨图像。本文介绍了自适应光学的起源与工作原理,总结了其在超分辨荧光显微镜中的应用,并展望了其未来的发展前景。
显微 荧光显微镜 超分辨 自动与自适应光学 像差补偿 波前传感
Youming Guo 1,2,3,4Kele Chen 1,2,3,4,5Jiahui Zhou 1,2,3,4Zhengdai Li 1,2,3,4[ ... ]Changhui Rao 1,2,3,4,*
Author Affiliations
Abstract
1 The Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 610209, China
2 Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China
3 University of Chinese Academy of Sciences, Beijing 100049, China
4 School of Electronic, Electrical and Commutation Engineering, University of Chinese Academy of Science, Beijing 100049, China
5 National Key Laboratory of Optical Field Manipulation Science and Technology, Chengdu 610209, China
Integrating deformable mirrors within the optical train of an adaptive telescope was one of the major innovations in astronomical observation technology, distinguished by its high optical throughput, reduced optical surfaces, and the incorporation of the deformable mirror. Typically, voice-coil actuators are used, which require additional position sensors, internal control electronics, and cooling systems, leading to a very complex structure. Piezoelectric deformable secondary mirror technologies were proposed to overcome these problems. Recently, a high-order piezoelectric deformable secondary mirror has been developed and installed on the 1.8-m telescope at Lijiang Observatory in China to make it an adaptive telescope. The system consists of a 241-actuator piezoelectric deformable secondary mirror, a 192-sub-aperture Shack-Hartmann wavefront sensor, and a multi-core-based real-time controller. The actuator spacing of the PDSM measures 19.3 mm, equivalent to approximately 12.6 cm when mapped onto the primary mirror, significantly less than the voice-coil-based adaptive telescopes such as LBT, Magellan and VLT. As a result, stellar images with Strehl ratios above 0.49 in the R band have been obtained. To our knowledge, these are the highest R band images captured by an adaptive telescope with deformable secondary mirrors. Here, we report the system description and on-sky performance of this adaptive telescope.
adaptive optics deformable secondary mirror visible imaging Opto-Electronic Advances
2023, 6(12): 230039
光子学报
2023, 52(12): 1201003
光子学报
2023, 52(11): 1111004